Tuesday 18 December 2018 16:00 – 17:00 Seminar Room 1
“Systems Biology approach for the elucidation of system-wide p53dependent signaling pathways in Hodgkin and Non-Hodgkin lymphomas towards efficient therapeutic strategies”
Dr. Konstantina Psatha Institute of Molecular Biology and Biotechnology (IMBB)
Lymphomas usually harbor a functionally inactive wild-type (wt) p53, promoting cell proliferation and survival, genomic instability and lymphoma resistance in therapy. Decoding the perturbed protein phosphorylation motifs contributing to lymphomagenesis is still required. The recently developed small molecule and p53-MDM2 antagonist, Nutlin-3a (N3a), induces a non-genotoxic reactivation of wtp53 protein, providing an excellent model system for the comprehensive understanding of p53 regulation, its effect on cell cycle arrest and apoptosis in lymphoma cells. In the present study, we used in vitro model lymphoma cell lines corresponding to different lymphoma subtypes +/- N3a for global comparative phosphoproteomic profiling, aiming to identify key phosphorylation events characteristic for every lymphoma (sub) type. The resulted datasets are being processed and evaluated by advanced bioinformatics tools, to conceptualize the first dynamic and detailed cell signaling map of lymphoma cells bearing a N3a-reactivated, offering detailed insights on the beneficial effect of N3a-mediated p53’s reactivation in the treatment of human lymphoma.